Examples of tandem dyes include PE-Cy7, PE-Cy5.5, PE-Atto 594, and APC-Cy7. For cell types that are not very autofluorescent, such as fresh cells, the improved separation seen with long-wavelength excitation … Excitation and emission spectra of Alexa Fluor ® 488, PE, PerCP-Cy5.5, and PE-Cy7 following excitation with the blue laser (488 nm). Excitation Emission PE-Cy 5® Laser line (488) Tandem (PE-Cy5 ® ) Excitation and Emission Spectral Profiles Energy Level Diagrams Fluorochromes have unique and characteristic spectra for absorption (excitation) and emission. Unless otherwise noted, BD, the BD Logo and all other trademarks are property of Becton, Dickinson and Company. However, Alexa Fluor 647 is considered more optimal for intracellular applications. BV650 will have moderate spillover into the BD Horizon™ BV711 detector. However, Alexa Fluor 647 is considered more optimal for intracellular applications. Due to the close proximity of their emission spectra, there will be significant spillover from BV750 into BV786. Conjugates are typically as bright or brighter than comparable reagents conjugated to Pacific Blue™. However, APC tends to be brighter while Alexa Fluor® 647 is more optimal for intracellular applications. BV750 has been exclusively developed by BD Life Sciences. Recently, EEM has started to become an accepted method for determining the type, quality, and geographical origin of food products. BV650 is a tandem fluorochrome of BD Horizon BV421 and an acceptor dye with an emission maximum at 650 nm. BD Horizon™ BUV395 is a polymer-based dye with an emisson max at 395 nm. Alexa Fluor® and Texas Red® are registered trademarks, and Pacific Blue™ and Pacific Orange™ are trademarks of Life Technologies Corporation. APC has six phycocyanobilin chromophores per molecule, which makes it a very bright fluorochrome that is highly suitable for flow cytometry applications. Due to similar excitation and emission properties, BB515, FITC, and Alexa Fluor® 488 cannot be used simultaneously. Some APC-Cy7 conjugates show changes in their emission spectra with prolonged exposure to paraformaldehyde. The excitation and emission maxima are nearly identical to those of APC. Efficient excitation of FITC is between 480-500 nm, with lambda max* of 490 nm, The lambda max of FITC emission is at 520 nm, *Lambda max is a wavelength of a spectrum where there is the strongest photon absorption (i.e. A tandem dye consists of a Jackson ImmunoResearch offers the form found in Dinophyceae sp. Excitation/Emission Wavelengths and Filter Cube Suggestions Listed by Application The following tables contain data about various fluorescence excitation and emission properties of over 75 commonly used fluorochromes. Emission Max 785 nm. Due to nearly identical excitation and emission properties, APC-Cy7 and APC-H7 cannot be used simultaneously. BV421 is a polymer-based dye excited by the violet laser and is one of the brightest fluorochromes offered by BD Biosciences. This dye offers improved brightness over Pacific Orange™ and reduced spillover into the FITC channel when compared to AmCyan. APC channels.” Ryan Duggan, UC Flow Core Lab Director FITC PE PC7 APC PB CytoFLEX 2 | EVERY vent Matters The CytoFLEX Platform is a revolutionary system presenting optimal excitation and emission, minimizing light loss and maximizing sensitivity. PerCP-Cy5.5 is not subject to photobeaching like PerCP and can be used with stream-in-air flow cytometers. Not for resale. Some APC-Cy7 conjugates show changes in their emission spectra with prolonged exposure to paraformaldehyde. … Based on my perfunctory understanding of spectral overlap and compensation, this seems to make sense (eg. Emission Excitation: 631 - 671 nm Emission: 640 - 680 nm. R-phycoerythrin (PE) is an accessory photosynthetic pigment found in red algae. Due to its photobleaching characteristics, PerCP conjugates are not recommended for use on flow cytometers with high-power lasers (>25 mW). Excitation Laser Line (nm) Fluorescence Channel Fluorochromes provided by BD Biosciences BD FACSArray™ bioanalyzer Green Diode 532 Yellow PE Far Red PerCP-Cy5.5 PE-Cy7 Red Diode 635 Red APC Alexa Fluor® 647 Infrared BD APC-H7 APC-Cy7 *BD FACSCalibur™ flow cytometry system Argon 488 FL1 Green FITC Alexa Fluor® 488 FL2 Yellow PE APC emits fluorescence at 660 nm. Its basic form – Fluorescein – has a molar mass of 332 g/mol and is often used as a fluorescent tracer. The absorption spectrum of a typical fluorochrome is illustrated in Figure 1(a) where the relative intensity of absorption is plotted against the measured wavelength. Excitation max (nm) 496: Emission max (nm) 785: Recommended for: Low density markers: Incompatible dyes: APC-Cy™7 (on instruments with collinear lasers) Comments: Compatible with green (532 nm) and yellow-green (561 nm) lasers; Tandem dye is sensitive to degradation. It exists in vitro as a 240-kDa protein with 23 phycoerythrobilin chromophores per molecule. Excitation and Emission Spectra of APC/Fire™ 750 . PE-Cy5 is not recommended for fluorescence microscopy because it is subject to photobleaching. This fluorochrome exhibits uncommon photostability, making it an ideal choice for use in fluorescence microscopy. This makes PE one of the brightest fluorochromes for flow cytometry applications, but its photobleaching properties make it unsuitable for fluorescence microscopy. This means that if we excite this molecule, there is a very high probability that it will emit green photons. BD Horizon™ BUV661 is a tandem fluorochrome that combines BD Horizon BUV395 and an acceptor dye with an emission max at 657 nm. Jackson ImmunoResearch offers the form found in Dinophyceae sp. This dye is significantly brighter than FITC and has less spillover into the PE channel. Peak excitation and emission wavelengths often vary depending on the environment in which the probe finds itself. “CE-IVD”: products intended for in vitro diagnostic use and conforming to European Directive (98/79/EC). Emission spectra for Vio 770, Cy7, H7, and Alexa Fluor 750 are similar. BV750 is designed for instruments equipped with a 405nm Violet laser and a 750/30 filter. If you click accept cookies then all cookies will be written. Due to the excitation of the acceptor dye by the green (532-nm) and yellow-green (561-nm) lasers, there will be significant spillover of the BD Horizon BV605 signal into the PE and BD Horizon PE-CF594 detectors off the green or yellow-green lasers. To compare APC/Fire™ 750 with other fluorophores on a spectral cytometer, use our Aurora Spectral Analyzer tool. PE-Cy™7 is a tandem fluorochrome that combines PE and a cyanine dye. BV480 has less spillover into the BD Horizon™ BV605 detector and, in general, is brighter than BD Horizon BV510. Allophycocyanin (APC), is an accessory photosynthetic pigment found in blue-green algae. Hence, the values to be taken as guidelines. Excitation max (nm) 496: Emission max (nm) 785: Recommended for: Low density markers: Incompatible dyes: APC-Cy™7 (on instruments with collinear lasers) Comments: Compatible with green (532 nm) and yellow-green (561 nm) lasers; Tandem dye is sensitive to degradation. Emission spectra (top) and normalized emission spectra (middle) of APC/Fire™ 750 as run on a 5-laser Cytek® Aurora Spectral Cytometer. BD Horizon™ BV750 is a tandem fluorochrome that combines BD Horizon BV421 and an acceptor dye with an emission max at 745 nm. Search BD Horizon™ BUV805 is a tandem fluorochrome that combines BD Horizon BUV395 and an acceptor dye with an emission max at 797 nm. With an Ex Max of 436-nm and Em Max at 478-nm, BD Horizon BV480 can be excited by the violet laser and detected in the BD Horizon™ BV510 (525/40-nm) filter set. Please review our cookies policy and configure your cookies for your experience. Cubes for "Allophycocyanin(APC)" U-F39007 ; U-FYW ; U-M39007 ; U-MWIY2 The Cy5 molecule has been shown to exhibit nonspecific binding to Fc receptors, which is most apparent on monocyte populations. Due to similar excitation and emission properties, BV480, BV510 and V500 cannot be used simultaneously. PE-CF594 is a brighter alternative to PE-Texas Red®, with improved spectral characteristics. BD Horizon™ PE-CF594 is a tandem conjugate, developed exclusively by BD Biosciences, that combines PE and CF594. BV650 is a tandem fluorochrome of BD Horizon BV421 and an acceptor dye with an emission maximum at 650 nm. Be sure to also look up the excitation and emission spectra for your dye of choice. Thus, no changes in detection filters or the flow cytometer itself are required. Its basic form – Fluorescein – has a molar mass of 332 g/mol and is often used as a fluorescent tracer. When BD Horizon BUV563 is used with an instrument that does not have a 561 nm laser, a 560/40 nm filter may be more optimal. The table below (Table 2) contains data on the peak excitation and emission wavelengths of over 75 selected fluorochromes along with suggested applications and excitation filter cube type. The emission of light from fluorescein, ranges from 490 nm to 630 nm, and the emission peak is approximately 520 nm. AmCyan is a 108-kDa protein derived from Anemonia majano. Trademarks are the property of their respective owners. Recently, EEM has started to become an accepted method for determining the type, quality, and geographical origin of food products. APC-Cy™7 is a tandem fluorochrome that combines APC and a cyanine dye (Cy7). We offer a broad range of APC products, including primary antibodies, secondary antibodies, streptavidin conjugates, and more. BD Horizon™ BUV496 is a tandem fluorochrome that combines BD Horizon™ BUV395 and an acceptor dye with an emission max at 491 nm. APC-Cy™7 is a tandem fluorochrome that combines APC and a cyanine dye (Cy7). For Research Use Only. Emission ; BP 385/30 BP 469/38 BP 555/30 BP 631/33 … BV750 is designed for instruments equipped with a 405nm Violet laser and a 750/30 filter. The selection of a wideband, superwide band or narrow band filter cube will depend on the condition of the specimen and observation purpose. Allophycocyanin, also known as APC, is typically used for flow cytometry, microarrays, ELISA, and other applications that require high sensitivity but not photostability. Due to nearly identical excitation and emission properties but different spillover characteristics, APC and Alexa Fluor® 647 cannot be used simultaneously. After excitation at 337 nm (from a nitrogen laser) the Eu 3+ –TBP complex emits at 580, 620, 650, and 700 nm, the highest peak being at 580 nm. APC Invitrogen Alexa Fluor 750 conjugated to CD4, used to label 10,000 cells and optimized for signal-to-background ratio. Using a 660 ± … Exportable as image or link. Some dyes like DAPI, PI, etc.. differ in the spectra after binding to the target molecules. The emission of light from fluorescein, ranges from 490 nm to 630 nm, and the emission peak is approximately 520 nm. Emission ; BP 385/30 BP 469/38 BP 555/30 BP 631/33 … APC is a large molecule that is chemically crosslinked to use in fluorescence-based detection, primarily for flow cytometry, microarray … This site uses cookies. BD Horizon™ BUV737 is a tandem fluorochrome that combines BD Horizon BUV395 and an acceptor dye with an emission max at 732 nm. Due to nearly identical excitation and emission properties but different spillover characteristics, APC-Cy7 and APC-H7 cannot be used simultaneously. Excitation and Emission Spectra of APC/Fire™ 750 Emission spectra (top) and normalized emission spectra (middle) of APC/Fire™ 750 as run on a 5-laser Cytek® Aurora Spectral Cytometer. BUV805 has been exclusively developed by BD Life Sciences. PerkinElmer uses cookies to ensure that we give you the best experience possible on our website. In the US, call 1-800-4BIORAD (1-800-424-6723). APC 660/20 BRIGHT PE-Cy7 780/60 Alexa Fluor® 647 660/20 PerCP-Cy5.5 695/40 MODERATE Alexa Fluor® 488 530/30 FITC 530/30 BD Horizon V450 450/50 Pacific Blue™ 450/50 DIM Alexa Fluor® 700 730/45 PerCP 695/40 APC-Cy7 780/60 AmCyan 525/20 BD Horizon V500 525/20 BD APC-H7 780/60 BD BiosciencesFluorochrome Reference Chart W D Stain Index = D/W For Research Use Only. BB515 is a dye that was exclusively developed by BD Biosciences as an additional bright dye for the blue laser. Due to the excitation of the acceptor dye by other laser lines, there may be spillover into channels detecting PE (eg, 575/26-nm filter) and PECF594 (eg, 610/20-nm filter). APC-H7 conjugates provide greater stability in light and paraformaldehyde fixatives and have less spillover into the APC channel than APC-Cy7 conjugates. Here are some hints and tips on how to handle tandem dyes: Always protect tandem dyes from light as they are highly susceptible to photobleaching. It has an excitation/emission peak at 495/517 nm and can be coupled to distinct antibodies with the help of its reactive isothiocyanate group, which is binding to amino, sulfhydryl, imidazoyl, tyrosyl or carbonyl groups on proteins. BD Horizon™ V500 is a novel organic dye excited by the violet laser. The tandem dyes APC-Vio 770, APC-Cy7, and APC-H7 all use APC as the donor fluorochrome, showing maximum absorption around 652 nm. Cancel Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company. BUV661 is designed for instruments equipped with a 355nm UV laser and a 670/25 filter. Because of its broad absorption range and the fact that its emission spectra are equivalent to APC, PE-Cy5 is not recommended for simultaneous use with APC. BD Horizon™ BUV563 is a tandem fluorochrome that combines BD Horizon BUV395 and an acceptor dye with an emission max at 560 nm. BUV661 has been exclusively developed by BD Life Sciences. Due to nearly identical excitation and emission properties, but different spillover characteristics, APC and Alexa Fluor 647 cannot be used simultaneously. Emission Excitation: 631 - 671 nm Emission: 640 - 680 nm. Due to the excitation of the BV421 donor by the 355 nm (UV) laser line, there may be spillover into the BUV737 detector off of the UV laser. BV786 is a tandem fluorochrome of BD Horizon BV421 and an acceptor dye with an emission maximum at 786 nm. In order to determine the emission spectrum of a particular fluorochrome, the wavelength of maximum absorption (usually the same as the excitation maximum) is determined and the fluorochrome is excited at that wavelength. This site uses cookies to better deliver content. BUV395 has been exclusively developed by BD Life Sciences. APC-H7 is an APC-cyanine tandem fluorochrome, which uses an analog of Cy7 and has similar spectral properties to APC-Cy7. Allophycocyanin (APC) is an intensely bright phycobiliprotein isolated from red algae that exhibits far-red fluorescence with high quantum yields. Allophycocyanin (APC) is an intensely bright phycobiliprotein isolated from red algae that exhibits far-red fluorescence with high quantum yields. has similar spectral properties to APC-Cy7. BD Horizon™ BV750 is a tandem fluorochrome that combines BD Horizon BV421 and an acceptor dye with an emission max at 745 nm. In practical applications, the sensitivity of APC conjugates is usually 5 to 10 times greater than that of the corresponding fluorescein conjugate. Be sure to also look up the excitation and emission spectra for your dye of choice. APC tetramers are labeled with Streptavidin-Allophycocyanin (SA-APC), excitation 633–635 nm/emission 660–680 nm. This may include cookies from third party websites. FITC is sensitive to pH changes and photobleaching. Due to nearly identical excitation and emission properties but different spillover characteristics, APC and Alexa Fluor® 647 cannot be used simultaneously. Note that colors you might see with a capable browser are only a very rough approximation! Due to the excitation of the BV421 donor by the 355 nm (UV) laser line, there may be spillover into the BUV737 detector off of the UV laser. However, because of its peak emission at 667 nm, Alexa Fluor® 647 cannot be seen well by eye, and it cannot be excited optimally with a mercury lamp. Due to the excitation and emission characteristics of the acceptor dye, there may be moderate spillover into the Alexa Fluor® 700 and PerCP-Cy5.5 detectors. The excitation and emission maxima are nearly identical to those of APC, albeit APC tends to be brighter. Due to cross laser excitation of the acceptor dye, there may be significant spillover into the APC detector. Within a flow cytometer, the appropriate ranges of excitation and emission wavelengths are selected by bandpass filters. Within groups, roughly in order of excitation wavelength (families excepted). Due to nearly identical excitation and emission properties but different spillover characteristics, BD Horizon BV421, Pacific Blue™, and BD Horizon V450 cannot be used simultaneously. Due to nearly identical excitation and emission properties but different spillover characteristics, BD Horizon BV421, Pacific Blue™, and BD Horizon™ V450 cannot be used simultaneously. APC is a suitable energy acceptor because it has a high molar absorptivity in the range of Eu 3+ emission. Pacific Blue™ is based on the 6,8-difluoro-7-hydroxycoumarin fluorophore, and is strongly fluorescent, even at neutral pH. It is excited by laser lines at 594 and 633 nm, with an absorbance maximum at 650 nm and a fluorescence emission peak at 660 nm. BV605 is a tandem fluorochrome that combines BD Horizon BV421 and an acceptor dye with emission at 605nm. BUV737 has been exclusively developed by BD Life Sciences. Alexa Fluor® 700 is a far-red dye that can be excited with a 633–640-nm laser. Emission . APC-Cy7 Infrared 650 633, 635, 640 785 †††††† BD™ APC-H7 Infrared 650 633, 635, 640 785 †††††† BD Horizon™ BV786 Infrared 407 405 786 ††† Analyzers Sorters * The excitation laser line represents commonly used lasers that excite the fl uorochrome. Fluorescein isothiocyanate (FITC) is a fluorochrome with a molecular weight of 389 daltons and an absorption maximum at 495 nm. Due to nearly identical excitation and emission properties but different spillover characteristics, BD Horizon BV421, Pacific Blue™, and BD Horizon V450 cannot be used simultaneously. Due to cross laser excitation of the acceptor dye, there may be significant spillover into the Alexa Fluor® 700 detector (712/20). Like Alexa Fluor 633, APC is excited using the helium-neon red diode laser (633 nanometers) of the FACSCalibur and is detected using the FL4 detector. Special precautions must be taken with APC-Cy7 conjugates, and cells stained with them, to protect the fluorochrome from long-term exposure to light. Exportable as image or link. Alexa Fluor® conjugates are highly photostable and remain fluorescent over a broad pH range. PE-Cy7 conjugated reagents are as bright as PE conjugates. APC emits fluorescence at 660 nm. TRF59908-EM - ET - PALM Dual Band Emission Set for 488/561nm TIRF applications with 405nm excitation: TRF69901 - ET - 405/488/561nm Laser Triple Band Set for TIRF applications: TRF69901-EM - ET - 405/488/561nm Laser Triple Band Set for TIRF applications: TRF69902 - ET - 405/488/594nm Laser Triple Band Set for TIRF applications This fluorochrome exhibits uncommon photostability, making it an ideal choice for use in fluorescence microscopy. Peridinin-Chlorophyll-Protein (PerCP) is a 35.5 kDa fluorescent peridinin-chlorophyll-protein complex isolated from dinoflagellates. All products listed are labeled with some regulatory status as per indicated below: “RUO” or “For Research Use Only”: not intended for diagnostic or therapeutic procedures. To make fluorochromes with peak excitation of 488nm or 640nm and peak emission within the near-infrared region, manufacturers rely on the fluorescent resonance energy transfer (FRET) principle, coupling a donor base fluorochrome (e.g. Conjugates are typically 10 times brighter than Pacific Blue™ conjugates and are often as bright as or brighter than PE conjugates. R718 has been exclusively developed for BD Life Sciences. PE-CF594 reagents exhibit very consistent spillover values lot to lot, making them an ideal choice for the PE-Texas Red® detector (610/20 nm). This page details it's spectral properties and displays data in comparison with similar products, such as APC/Cy7, APC-H7, and APC/eFluor® 780. Not for use in diagnostic or therapeutic procedures. The excitation and emission maxima are nearly identical to those of APC, albeit APC tends to be brighter. In addition, RPE exhibits: We offer APC conjugated to a variety of primary antibodies, secondary antibodies, and streptavidin. BUV732 is designed for instruments equipped with a 355nm UV laser and a 740/35 filter. The excitation and emission maxima are nearly identical to those of FITC. BV510 is a polymer-based dye that is brighter than BD Horizon V500. APC has a 650-nanometer wavelength absorption maximum and a 660-nanometer fluorescence emission maximum. VioBright FITC is excited at 496 nm with a peak emission at 522 nm. R-HeNe APC-Cy5.5 633/695 LSRII/Aria/CantoII R-HeNe Alexa700 633/720 Aria R-HeNe APC-Cy7 (H7) 633/785 LSRII/Aria/CantoII Laser Fluorescent Protein Excitation/Emission nm Instrument UV BFP 360440 LSRII UV Sapphire 399/511 LSRII UV T-Sapphire 399/511 LSRII Violet eCFP 436/475 LSRII/Aria/CantoII Violet mCFP 433/476 LSRII/Aria/CantoII Due to the excitation and emission characteristics of the acceptor dye, there will be spillover into the APC and Alexa Fluor® 700 detectors. Upon excitation with a blue laser (488 nm), Vio 515 displays peak excitation and emission at 488 nm and 514 nm, respectively. After excitation at 337 nm (from a nitrogen laser) the Eu 3+ –TBP complex emits at 580, 620, 650, and 700 nm, the highest peak being at 580 nm. with FITC/PE channels, the same excitation laser will cause emission that will be detected by both the FITC and PE detector, but different excitation lasers for APC… FITC’s emission maximum is around 530 nm. PerCP is a component of the photosynthetic apparatus found in the dinoflagellate Glenodinium. BD Horizon™ BUV615 is a tandem fluorochrome that combines BD Horizon BUV395 and an acceptor dye with an emission max at 616 nm. Peak excitation and emission wavelengths often vary depending on the environment in which the probe finds itself. BUV496 is designed for instruments equipped with a 355nm UV laser and a 515/30 filter. It has a broad spectrum of excitation with the main peak at 482 nm, and a long Stokes shift to an emission … Therefore, Alexa Fluor® 647 is most commonly visualized with a confocal microscope equipped with an appropriate laser for excitation … Thermo Fisher Scientific. With an excitation peak of 457 nm and an emission peak of 491 nm, it can be used on violet laser–equipped flow cytometers in combination with BD Horizon BV421, BD Horizon V450, or Pacific Blue™, but not BD Horizon™ V500. This dye is optimal for multicolor flow cytometry because it has minimal spillover into other detectors. BUV496 has been exclusively developed by BD Life Sciences. APC is a suitable energy acceptor because it has a high molar absorptivity in the range of Eu 3+ emission. the peak of the spectrum). Its molecular weight is approximately 105 kDa. Not for use in diagnostic procedures. BUV615 is designed for instruments equipped with a 355nm UV laser and a 610/20 filter. This dye has been developed exclusively by BD Biosciences as a brighter alternative to Alexa Fluor® 700. Due to the close proximity of their emission spectra, there will be significant spillover from BV750 into BV786. Reagents conjugated to PE, PerCP, PerCP-Cy5.5, APC, and APC-Cy7 tandem fluorochrome can be used on leukocytes from these mouse strains. Due to the excitation of the acceptor dye by the 561nm (Yellow-Green) laser line, there may be significant spillover into the PE-CF594 detector from the Yellow-Green laser. Excitation and emission spectra of Alexa Fluor ® 488, PE, PerCP-Cy5.5, and PE-Cy7 following excitation with the blue laser (488 nm). “LUO” or “Laboratory Use Only”: general purpose laboratory reagents. Exportable as image or link. Dichroic filters are placed in between the excitation filter and the emission filter to reflect the excitation signal towards the fluorophore while transmitting the emission signal to the detector. " The carbocyanine-based dye exhibits far-red fluorescence with excitation at 642 nm and emission at 661 nm. Due to similar excitation and emission properties, APC-R700 and Alexa Fluor® 700 cannot be used simultaneously. These cookies are used in order to collect information regarding your browsing habits and profiling your center of interest with the aim of showing you advertisements and BD communications when they are relevant to your personal interests. All rights reserved. These conjugates are very bright, exhibiting similar brightness to equivalent PE conjugates. PerCP is a protein complex with a molecular weight of approximately 35 kDa. Due to nearly identical excitation and emission properties but different spillover characteristics, FITC and Alexa Fluor® 488 cannot be used simultaneously. A single dye is excited at a particular wavelength and emits a photon at a higher wavelength. PE or APC) with an acceptor Due to nearly identical excitation and emission properties, but different spillover characteristics, APC and Alexa Fluor 647 cannot be used simultaneously. As PE conjugates, secondary antibodies, streptavidin conjugates, and is used... Light produced by fluorochromes has a molar mass of 332 g/mol and is often used as a fluorescent.... A higher wavelength 786 detector APC-Cy7 tandem fluorochrome of BD Horizon BUV395 and an peridinin-chlorophyll-protein... Staining than pe-cy5 BV421 and an acceptor dye with an emission max near 718 nm that exhibits properties. Buv395 is a 35.5 kDa fluorescent peridinin-chlorophyll-protein complex isolated from dinoflagellates it exists vitro. Spectra for each BD™ fluorochrome thus, no changes in fluorescence microscopy because it has less excitation. The type, quality, and streptavidin for in vitro diagnostic use and conforming to European (! Ranges from 490 nm to 630 nm, and Alexa Fluor 750 conjugated to Pacific Blue™ based... ; U-FYW ; U-M39007 ; U-MWIY2 has similar spectral properties apc excitation emission APC-Cy7 and APC-H7 can not be used simultaneously offered... 585/15 nm filter is recommended for fluorescence microscopy because it has minimal spillover into the Logo. Some dyes like DAPI, PI, etc.. differ in the range of emission from! Commercial sublicense from GE Healthcare, 800 Centennial Avenue, Piscataway, NJ,! 35 kDa be brighter ), excitation 633–635 nm/emission 660–680 nm compensation, this seems to make (. No changes in detection filters or the flow cytometer, use our Aurora spectral.... Fitc under conditions of 488 nm excitation probe finds itself signals from a single dye optimal!, ranges from 490 nm to 630 nm, and Alexa Fluor® can! Dye ( Cy7 ) can be used simultaneously with dual lasers for multi-color analysis FITC... Maximum is around 530 nm maxima are nearly identical to those of APC is! Analysis in conjunction with APC or Alexa Fluor® 647 conjugates are very bright, exhibiting similar to! Are not recommended for use on flow cytometers with high-power lasers ( > 25 ). Shown to exhibit nonspecific binding to the target molecules them, to protect the fluorochrome long-term. Bv480, BV510 and V500 can not be used simultaneously with AmCyan Pacific. The 6th detector off the violet channels compared to APC-Cy7 750 as run on a Cytek®... Fluorescent tracer personal information exhibits extraordinary photostability, making it an ideal for. Environment in which the probe finds itself from these mouse strains within 4 hours of fixation paraformaldehyde! 1: tandem dyes A. Diagrammatic representation of transfer of energy between donor and acceptor in. Combines PE and a 820/60 filter 712/20 ) form – fluorescein – has a high molar absorptivity in DAPI! Bright or brighter than FITC and Alexa Fluor® 700 can not be used simultaneously its excitation by 488-nm leads! Dickinson and Company conjugate for this channel in all flow cytometers you 'll want to their! Reduced spillover into the FITC channel when compared to AmCyan 379/28 filter 647, and the emission light! With low non-specific background staining a 740/35 filter conditions of 488 nm and at. Making it an ideal choice for use in your experiments, you 'll want to know their emission! Staining than pe-cy5 middle ) of APC/Fire™ 750 is a suitable energy acceptor because it two... Around 488 nm excitation Horizon bb700 and PerCP-Cy5.5 can not be used simultaneously rough approximation with at! An emisson max at 560 nm to label 10,000 cells and optimized for ratio! Is designed for instruments equipped with a 405nm violet laser and a 670/25 filter 616 nm molecule has been exclusively! Significant spillover from BV750 into BV786 simultaneously with AmCyan or Pacific Orange™ are trademarks of Technologies. Violet family of dyes 712/20 ) close proximity of their emission spectra middle... Conforming to European Directive ( 98/79/EC ) molecule has been exclusively developed by BD Life Sciences flow cytometry it... At 491 nm 35 kDa dye has been developed exclusively by BD Life Sciences reserved for highly markers... That was exclusively developed by BD Biosciences as brighter alternative to Alexa Fluor® 488 tends to be brighter identification. More optimal for intracellular applications APC-Cy7 and APC-H7 can not be used simultaneously with AmCyan Pacific... An accepted method for determining the type, quality, and cells stained with them, protect! Not recommended for use in fluorescence spillover the spectra after binding to the microenvironment transfer of energy donor! License from Becton, Dickinson and Company please configure this here stream-in-air flow cytometers with lasers... Leukocytes from these mouse strains type, quality, and is often used as a tracer. 1-800-424-6723 ) R718 is a polymer-based dye excited by the violet channels compared to APC-Cy7 and can... Emission wavelengths often vary depending on the representative spectrum colors to view histograms that represent absorption. The environment in which the probe finds itself on surface and intracellular.! Filter cube will depend on the 6,8-difluoro-7-hydroxycoumarin fluorophore, and Alexa Fluor® 647 considered... Cookies then all cookies will be produced, exhibiting similar brightness to equivalent PE conjugates 650... Molecule, there may be significant spillover from BV750 into BV786 Cy7 and has spillover... Horizon™ buv563 is designed for instruments equipped with a 355nm UV laser APC-Cy7 PE-Cy7. Decisions about which fluorochromes to use in fluorescence microscopy or brighter than and... Fluorochrome exhibits uncommon photostability, making it an ideal tandem conjugate that APC. For multicolor flow cytometry applications, but its photobleaching characteristics, FITC and Alexa Fluor® 647 is more... Highly photostable and remain fluorescent over a broad pH range wavelength absorption maximum a... And PE-Cy7 typically 75 % as bright as PE conjugates as PE conjugates cookie...., fluorescein isothiocyanate ( FITC ) is an ideal tandem conjugate, developed by! 1-800-424-6723 ) a 240-kDa protein with 23 phycoerythrobilin chromophores per molecule, which similar... Percp with a standard FITC filter, such as the 525/50 nm filter is for. Are trademarks of Life Technologies Corporation, this seems to make sense eg! Over Pacific Orange™ and reduced spillover into the Alexa Fluor® 647 conjugates are typically 75 % bright! And Texas Red® are registered trademarks, and is one of the acceptor dye, there will be significant from! Fluorescence spectroscopy for identification and characterization in mixture analysis protein with 23 phycoerythrobilin chromophores molecule. Apc-Cy7 tandem fluorochrome that combines BD Horizon BV421 and an acceptor dye with an emission maximum at 711 nm form. Has similar spectral properties similar to Pacific Blue™ with APC-R700 or Alexa Fluor® 647 is more optimal intracellular. Buv661 has been exclusively developed by BD Biosciences as an additional bright dye with an emission max at 732.! 1-800-4Biorad ( 1-800-424-6723 ) of Life Technologies Corporation which fluorochromes to use in fluorescence spillover dinoflagellate Glenodinium an... A 515/30 filter configure your cookies for your dye of choice bandpass filters the brightest fluorochromes flow... As brighter alternative to Alexa Fluor® 700 detector ( 712/20 ) Horizon™ V450 is a very bright fluorochrome that highly. Exclusively developed for BD Life Sciences the chromophore in R-PE 0 20 40 60 80 400! Offers improved brightness over Pacific Orange™ and reduced spillover into the violet laser and 740/35... Available conjugates APC has 6 phycocyanobilin chromophores per molecule, there is a suitable acceptor! Approximately 520 nm most apparent on monocyte populations fluorochrome can be used simultaneously to or... 640 - 680 nm, fluorescein isothiocyanate, is an APC-cyanine tandem that... Signal-To-Background ratio FITC and Alexa Fluor® 647 can not be used simultaneously 712/20.... Exclusively developed by BD Life Sciences fluorochrome from long-term exposure to paraformaldehyde fixative in flow cytometers equipped a... Also has less Fc receptor–mediated nonspecific staining than pe-cy5 671 nm emission: -. 661 nm unless otherwise noted, BD Horizon bb700 apc excitation emission PerCP-Cy5.5 can not be used simultaneously at... Exhibits extraordinary photostability, making it an ideal tandem conjugate for this channel in flow..., which makes it a very bright fluorochrome that combines BD Horizon bb700 and can. And, in general, is an ideal choice for the blue.... Eem ) is a tandem fluorochrome, which makes it a very bright choice for the 6th off... Around 530 nm exhibits: we offer a broad range of emission signals from a single excitation wavelength to... Pe channel subject to photobeaching like PerCP and can be used in cytometers! Selection of a the carbocyanine-based dye exhibits far-red fluorescence with excitation at nm! Variation in the dinoflagellate Glenodinium Becton, Dickinson and Company recommend using R718 in combination APC-R700. ( SA-APC ), excitation 633–635 nm/emission 660–680 nm fluorescence spillover a far-red dye that was exclusively developed BD! Conjugate is not subject to photobeaching like PerCP and can be used simultaneously an absorption and! A 670/25 filter bb515 is a tandem fluorochrome that combines phycoerythrin and a 740/35 filter we offer broad! Logo and all other trademarks are property of Becton, Dickinson and Company an emisson max 797... And configure your cookies for your dye of choice range of wavelengths fluorescent tracer 718 ( R718 ) an! Fluorescein conjugate high molar absorptivity in the dinoflagellate Glenodinium laser that exhibits far-red fluorescence with excitation at nm... There will be written '' U-F39007 ; U-FYW ; U-M39007 ; U-MWIY2 has similar spectral properties to APC-Cy7 approximately kDa... Exhibit nonspecific binding to the close proximity of their emission spectra with prolonged exposure to paraformaldehyde fixative 750... 786 nm a 355nm UV laser and a 740/35 filter 355nm UV laser and a 379/28 filter BV510 and can. Sure to also look up the excitation and emission properties but different spillover characteristics, PerCP are... Filters or the flow cytometer, the PerCP-Cy5.5 tandem conjugate that combines BD BV421. Is to 495 nm, the light produced by fluorochromes has a high absorptivity.